Friday, June 11, 2010

Listen to what plants say...

American Society for Plant Biologists.
31 July - 4 August
Montrèal, Canada

Following is the collection of Peroxisome related talks and posters from this future event

- Talks -

Minisymposium - Organelle Biology
3 Aug 2010, 9:20AM
Kyaw Aung (Student of Jianping Hu lab, Michigan state uni.)
An Arabidopsis C-terminal tail-anchored protein in peroxisomal and mitochondrial division and positioning.
Authors have identified a novel Plant specific protein, Peroxisomal and Mitochondrial Division Factor1 (PMD1) which is C-terminally tail anchored on outer membrane of peroxisomes and mitochondria whereas N-terminus contains four coiled-coil domains. PMD1 Null mutant contains enlarged peroxisomes and elongated mitochondria whereas over-expression leads to aggregated peroxisomes and mitochondria.  Since PMD1 did not interact with Pex11, Fis1 and Drp3, it is speculated that PMD1 follows completely novel pathway in organelle maintenance.

Minisymposium 19. Enzyme Structure & Function
3 Aug 2010 10:40 M
Acyl CoA oxidase catalyse first step in peroxisomal fatty acid beta-oxidation. The authors have studied different acx mutants and characterized the biochemical activities and respective phenotypes.

- Posters -

Indole-3- Butyric Acid response mutant 3: Dehydrogenase in the Peroxisome?
Robert Barlow (Bethany K. Zolman Lab)
Indole-3-Acetic Acid (IAA) is a plant hormone. Authors observed that conversion of Indole-3-butyric Acid (IBA) to IAA occurs in peroxisomes in a process resembling fatty acid beta oxidation. Authors identified in screen, IBR3, a protein having Acyl CoA dehydrogenase activity as well as aminoglycoside phosphotransferase domain and peroxisomal targeting signal.

Rapid organelle responses to reactive oxygen species
Jaideep Mathur
The authors have characterized morphological changes in peroxisomes and mitochondria in response to ROS stress. Peroxules and matrixules are tubular extentions of peroxisome and mitochondria. Photo-convertible EosFP PTS1 was used to study peroxisome morphology and dynamics. Prolonged ROS stress causes peroxule and matrixule inhibition and leads to asynchronous, asymmetric fission mediated organelle division.

Systemic phenotypic analysis of mutants of novel peroxisomal genes in Arabidopsis
Gaëlle Cassin (Post-doc in Jianping Hu Lab)
Authors have identified over 50 Arabidopsis peroxisomal proteins by proteomics and bioinformatics approach. Authors will present the characterization of T-DNA insertion mutants of these genes and their biochemical and phenotypic outcomes.

Suppression of peroxisome biogenesis factor 10 reduces cuticular wax accumulation by disrupting the ER network in Arabidopsis thaliana.
Kamigaki Akane
Pex10 RNAi in Arabidopsis leads to defects in ER morphology, cytosolic mislocalization of ER resident proteins involved in wax biosynthesis CER1, CER4, WAX2. (The paper is already published in Plant and Cell Physiol., interestingly this seems first and sole report of Pex10 affecting ER.)

Transcriptome Analysis Reveals the Involvement of Genes from Different Metabolic Pathways during Adventitious Root Formation (ARF) in Petunia
Amirhossein Ahkami
I found the abstract very interesting. Adventitious roots are essential for establishing new plantlet from parts cut from mother plant. Transcritome analysis identified more than 3500 genes significantly induced in various phases of ARF. Activation of peroxisomal beta oxidation was observed by authors and speculated to be involved in lipid catabolism and plant hormones like Jasmonic acid and IAA.

Isotopic Signature of Photorespiration
Dianne Pater
Authors are characterizing stoichiometric changes in CO2 release and O2 uptake in Peroxisomal malate dehydrogenase (PMDH) mutants as well as from Wild type.

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