Monday, July 5, 2010

Peroxisome Abstracts from Future..

SEB Annual Main Meeting 2010
Clarion Congress Hotel, Prague, Czech Republic
30th June - 3rd July 2010

Peroxisome related abstracts in poster session

From the session - Plant Cell Biology: Cell Biology of Plant Defense

Poster Session 1st July 2010 (Thursday) 17:00 – 19:00

Cross-kingdom characterization of peroxisomal ABC transporters
Xuebin Zhang (Rothamsted Research) et al. 
The authors have performed heterologous expression of mammalian ABC transporter proteins adrenoleukodystrophy protein (ALDP), ALD related protein, PMP70 and P70R which are properly sorted to peroxisomes(ALDP,ALDRP,PMP70) and ER(P70R) in plants(tobacco epidermis cells). They observed that Arabidopsis Pex19_1 can interact with both plant as well as human ABC proteins. The major observation by the authors is that disease causing mutant human ALDP proteins which are either degraded or mislocalized in human cells, are still properly targeted to peroxisomes in plants upon heterologous expression and are stable. 

Novel chemical inhibitors provide fresh insights into peroxisome form and function 
Laura-Anne Brown (University of Leeds) et al. 
Different yet elegant approach is being used by Dr. Alison Baker group to gain better understanding of peroxisome biogenesis in plants. To overcome the limitations of lethality or redundancy in classical genetics approach, the authors are using chemical genetics approach to screen small molecule compounds which perturb peroxisome biogenesis by mislocalizing peroxisome directed GFP reporter. The authors have come up with two compounds, one being potent inhibitor of peroxisomal protein import while other inhibits peroxisomal β-oxidation. 

Dissecting the peroxisomal protein import pathway using novel small molecule probes 
Catherine O’Leary-Steele (University of Leeds) et al. 
Authors present the data about the chemical genetic screen for the compounds disturbing peroxisomal protein import in plants. Authors identified three small molecule inhibitors of peroxisomal import affecting both PTS1 as well as PTS2 proteins. Authors are optimizing these lead compounds to reduce the cytotoxicity, and finding the binding partners using surface Plasmon Resonance analysis. The efforts are also going to identify the interactors (peroxisome membrane fraction proteins) by pull down experiments and proteomics.

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