Tuesday, March 29, 2011

A Novel micropexophagy specific protein 'Atg35' identified in Pichia pastoris

Atg35, a micropexophagy-specific protein that regulates micropexophagic apparatus formation in Pichia pastoris
Nazarko VY et al,
Autophagy, Volume 7, Issue 4,   April 2011, Pages 375 - 385

Pexophagy can occur by two distinct pathways termed as macropexophagy and micropexophagy. In Pichia pastoris, methanol to ethanol shift causes macropexophagy while methanol to glucose shift results in peroxisome degradation by micropexophagy.

During micropexophagy, peroxisome cluster is first step-wisely surrounded by engulfing vacuole and requires a specific component called micropexophagy apparatus (MIPA) for the complete engulfment and subsequent degradation.

Using Atg28 as a bait in Yeast two hybrid screen, Authors identified a novel protein Atg35 which is micropexophagy-specific and important for efficient MIPA formation. Overexpression or deletion of this protein abolished MIPA formation without affecting other autophagic pathways. It is also observed that Atg28 bridges the interaction between Atg17 and Atg35. Authors also characterized the novel site PNS (Perinuclear Structure) where Atg35 is recruited by Atg17.

More pexophagy related recent literature -

CCZ1, MON1 and YPT7 genes are involved in pexophagy, the Cvt pathway and non-specific macroautophagy in the methylotrophic yeast Pichia pastoris
Cell Biol Int. 2011 Feb

The phosphoinositide 3-kinase Vps34p is required for pexophagy in Saccharomyces cerevisiae
Biochem J. 2011 Jan

A yeast MAPK cascade regulates pexophagy but not other autophagy pathways
J Cell Biol. 2010 Apr

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