Saturday, March 26, 2011

Novel yeast Peroxin identified - Pex34

The Peroxin Pex34p Functions with the Pex11 Family of Peroxisomal Divisional Proteins to Regulate the Peroxisome Population in Yeast
Robert J. Tower, Andrei Fagarasanu, John D. Aitchison, and Richard A. Rachubinski
Molecular Biology of the Cell 2011, doi:10.1091/mbc.E11-01-0084

Authors have characterized the protein encoded by YCL056c gene. Currently the protein is annotated as "Protein of unknown function" in Saccharomyces Genome Database and described as green fluorescent protein (GFP)-fusion protein localizing to the cytoplasm in a punctate pattern. (schematic image from SGD)

Authors have renamed the protein as Pex34 which is an integral peroxisomal membrane protein. Pex34 is found to be positive regulator of peroxisome population under constitutive as well as inducing conditions. Pex34 deletion led to reduced number and enlarged peroxisomes whereas overexpression led to increased peroxisome number. Authors also found interaction of pex34 with itself and other Pex11 family peroxins using yest two hybrid analysis. Notably Pex34 interacts with Fis1 but not with Vps1.

Note - Recently Pex33 was identified from Neurospora crassa
Identification of PEX33, a novel component of the peroxisomal docking complex in the filamentous fungus Neurospora crassa
Managadze D et al.

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