Tools

A Novel Fluorescent Sensor Protein for Visualization of Redox States in the Cytoplasm and in Peroxisomes
Yano T. et al.
Mol. Cell. Biol. Accepted Article

High-Quality Immunofluorescence of Cultured Cells
Protein Secretion
Methods In Molecular Biology
Volume 619


Discrimination between alternate membrane protein topologies in living cells using GFP/YFP tagging and pH exchange
Domingo B et al,
Cell Mol Life Sci. 2010 May 8.
For many peroxisomal membrane proteins, conclusive topology is not yet known. The innovative strategy described in this paper can be utilized for determining the topology of membrane protein in any organellar or cellular membrane.

Enhanced Beetle Luciferase for High-Resolution Bioluminescence Imaging
Nakajima et al.
PLoS One. 2010; 5(4): e10011
Figure from the paper describing the use of the construct to label mammalian peroxisomes


Two-color Photoactivatable Probe for Selective Tracking of Proteins and Cells
JBC, April 2010
                  The authors have developed Photoactivatable Green Cherry (GPAC) which can be used to track the protein or  organelle in a cell, tissue and even in an organism!! The “continuously red-photoactivatable green” as authors call it, is advantageous in analyzing the dynamic changes in localization associated with various cellular stimuli.